HYDRAGEL 11 VON WILLEBRAND MULTIMERS

Simplified, same-day results, von Willebrand multimers gel assay

Test presentation

HYDRAGEL von WILLEBRAND MULTIMERS kit is designed for the detection of abnormalities in the VWF multimers distribution related to a hemorrhagic diathesis.
The test allows to assess the overall size distribution of von Willebrand factor multimers in human plasma using agarose gel electrophoresis and immunofixation, with the semi-automated the HYDRASYS 2 instrument.
HYDRAGEL von WILLEBRAND MULTIMERS assay provides within-day results, thanks to its simplified procedure, reduced hands-on time, ready-to-use reagents, and clear and reproducible profiles.

 

Test principle

HYDRAGEL von WILLEBRAND MULTIMERS allows separating small, intermediate and large multimers. The assay is carried out in two stages:

  • Sample pre-treatment followed by an electrophoresis done in SDS agarose gel to separate proteins according to their molecular weight,
  • direct immunofixation of multimers in the gel using anti-human vWF antibody followed by immunoenzyme staining to visualize the different Multimers.
    The semi-automated HYDRASYS 2 instrument performs all the steps needed to obtain gels ready for visual and densitometry-based interpretations.

Performances

  • Same-day results
  • Ready-to-use gels and reagents
  • Excellent reproducibility
  • Reduced hands-on time
  • All multimer sizes
  • Densitometry helps for results interpretation
  • Patient's profile overlayed with a normal control

Kit description

HYDRAGEL 11 von WILLEBRAND MULTIMERS kit (PN 4360): 10 gels of 11 tests to be associated with 2 revelation kits (PN 4747)

Kit content:

  • Agarose gels
  • Buffered strips
  • Sample diluent
  • Wash solution
  • Intermediate wash solution
  • Rehydrating solution
  • Plastic masks
  • Thin filter Papers
  • Thick filter Papers

5 von WILLEBRAND MULTIMERS VISUALIZATION kit (PN 4747): 2 kit are necessary for 10 gels of 11 tests associated to electrophoresis kit (PN 4360)

Kit content:

  • Antiserum diluent
  • Anti-von Willebrand factor antiserum
  • Anti-Ig G - PER antibody
  • TTF1 / TTF2 solvent
  • TTF1 (stock solution)
  • TTF2 (stock solution)

Related products and accessories not provided in the kit:

  • HYDRASYS 11 von WILLEBRAND MULTIMERS ACCESSORIES SEBIA (PN 1653)
  • METAL WEIGHT 2.3 KG SEBIA (PN 1654)

 

Compatible Instruments

HYDRASYS 2

HYDRASYS 2 SCAN

HYDRASYS 2 SCAN FOCUSING

See all the posters and publications related to VWF in the SCIENTIFIC PUBLICATIONS section.

For Research Use Only.

Contact your Sebia representative for more details.

Other products and publications associated