The Sebia systems use the principle of capillary electrophoresis (CE) in free solution.
Charged molecules are separated by their electrophoretic mobility at a specific pH in an alkaline buffer.
Separation occurs according to the electrolyte pH and electroosmotic flow. CE has a steady flow, compared to the pumped parabolic flow of the High-Performance Liquid Chromatography technique (HPLC). The steady flow results in narrower peaks and better resolution.
The Sebia capillary electrophoresis instruments are equipped with several parallel capillaries enabling multiple simultaneous analyses and scalable throughput: