Fully automated immunological identification of monoclonal proteins by capillary electrophoresis for small to medium size laboratories

Test presentation 

MINICAP IMMUNOTYPING kit allows the immunological identification of monoclonal components in human serum or urine. This test is fully automated on MINICAP instruments.

MINICAP instruments perform all sequences automatically, from sample dilution to migration, in order to obtain protein profiles for qualitative analysis. Profiles are visually interpreted to screen for any pattern abnormalities.

The combination of MINICAP IMMUNOTYPING kit with high resolution MINICAP PROTEIN(E) 6 buffer allows the detection and identification of monoclonal components.


Test principle

The MINICAP IMMUNOTYPING assay is based on the principle of capillary electrophoresis in free solution. Serum and urine proteins are separated in silica capillaries by their electrophoretic mobility and electroosmotic flow at high voltage in an alkaline buffer. Proteins are directly detected during migration by UV absorbance.

Serum and urine proteins are mixed with specific antisera directed against:

  • Gamma- (IgG),  Alpha- (IgA),  Mu- (IgM) heavy chains
  • Kappa (κ) and Lambda (λ) free and bound light chains

Immunoglobulins specifically react with their corresponding antiserum. At the end of the analysis, each antiserum pattern (IgG, IgA, IgM, κ and λ) is automatically overlaid with the ELP (Protein Electrophoresis) curve.

Disappearance of the abnormality in the antiserum-treated pattern indicates the presence of a monoclonal protein.


  • Throughput: 2 immunotyping tests/h
  • Sensitivity:
    • Internal study has demonstrated that a monoclonal protein can be detected at a concentration as low as 25 mg/dL in serum and 2.5 mg/dL in urine. According to the mobility of the monoclonal component and polyclonal background, the sensitivity may vary.
  • Full traceability and positive ID for samples and reagents 

Kit description

MINICAP IMMUNOTYPING (PN 2300) kit content:

  • Sample diluent vials
  • Rack with ELP solution and antiserum tubes:
    • Color-coded anti-IgG, -IgA, -IgM, -κ (free and bound), –λ (free and bound)​
    • 60 immunotyping tests

Related products and accessories not provided in the kit:

  • Quality controls:
    • Normal control serum (PN 4785)
    • Hypergamma control (PN 4787)
    • IT/IF control (PN 4788)
  • MINICAP PROTEIN(E) 6 kit (PN 2203)
  • CAPILLARYS/MINICAP URINE dialysis buffer (PN 2013)
  • SEBIA dialysis system (PN 9200)
  • FLUIDIL (PN 4587)
  • CAPICLEAN (PN 2058) - only for MINICAP instrument
  • CAPICLEAN MINICAP (PN 2251) - only for MINICAP FLEX-PIERCING instrument
  • CAPIprotect (2 x 5L) (PN 2061)

Compatible Instruments



* For serum only

NOTE: Contact your Sebia representative to verify the product availability in your country.