von Willebrand disease (VWD), can be divided into three major types(1):
People with VWD experience frequent nosebleeds, easy bruising, and excessive bleeding during and after invasive procedures, such as tooth extractions and surgery. Women often experience heavy menstrual bleeding (heavy menstrual periods that last longer than average) and haemorrhaging after childbirth.
The choice of an appropriate therapy depends on VWD subtyping and severity.
The diagnosis and typing of VWD requires the combination of several specialized tests. VWF multimers analysis is the only test allowing to detect abnormalities in multimers distribution, important for VWD subtyping and for finding the etiology of acquired VWD. Using electrophoretic protein separation, VWF multimers can be separated into following classes based on their molecular weight (MW): low (LMWM), intermediate (IMWM) and high MW (HMWM).
The VWF multimeric method based on luminographic detection,(2,3) is considered the “gold standard” for diagnosis VWD but the VWF multimers analysis on-homemade gels is a time consuming and technically challenging method, without standardization and available only in few specialized laboratories.
Thanks to our strong know-how in gel electrophoresis techniques, Sebia commercialized the VWF multimers analysis to significantly improve the efficiency of laboratories performing VWF analysis.
HYDRAGEL von WILLEBRAND MULTIMERS assay allows to assess the overall size distribution of von Willebrand factor multimers in human plasma using agarose gel electrophoresis and immunofixation, with the semi-automated the HYDRASYS 2 instrument.
Algorithm for congenital and acquired von Willebrand disease includes Hydragel vWF multimer analysis.
Multimer analysis to improve von Willebrand disease classification with Hydragel vWF multimer Sebia.
Correlation of von Willebrand factor with the severity of COVID-9 progression.
There are three types of von Willebrand Disease. In this leaflet, different cases from congenital and acquired VWD are presented.
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Multimer assay simplified.
(1) Sadler JE, Budde U, Eikenboom JC, Favaloro EJ, Hill FG, Holmberg L, et al. Update on the pathophysiology and classification of von Willebrand disease: a report of the Subcommittee on von Willebrand Factor. J Thromb Haemost. 2006;4:2103–14.
(2) Budde U, Schneppenheim R, Plendl H, Dent J, Ruggeri ZM, Zimmerman TS. Luminographic detection of von Willebrand factor multimers in agarose gels and on nitrocellulose membranes. Thromb Haemost. 1990;63:312–5.
(3) Schneppenheim R, Plendl H, Budde U. Luminography–an alternative assay for detection of von Willebrand factor multimers. Thromb Haemost. 1988;60:133–6.
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