von Willebrand
Multimer Analysis

Multimer assay simplified.

About von Willebrand Multimer Analysis

von Willebrand disease (vWD) is a genetic bleeding disorder caused by missing or defective von Willebrand factor (vWF).
Since von Willebrand disease (vWD) is a complex disease, its diagnosis requires a large panel of tests for an accurate diagnosis and classification of vWD. Multimer analysis is an integral part of the diagnostic process and an essential information for the clinicians for an optimal patient management.

HYDRAGEL von WILLEBRAND MULTIMERS kit is designed for the detection of abnormalities in the vWF multimers distribution related to a haemorrhagic diathesis. The test allows to assess the overall size distribution of von Willebrand factor multimers in human plasma using agarose gel electrophoresis and immunofixation, with the semi-automated HYDRASYS 2 instrument.

Key benefits

Semi-automated test

providing standardised and reproducible results.

Simplified technique

making this test accessible to any laboratory.

Within-day results

compared to in house tests delivering results in 4-5 days.

Traceability of the result

with densitometry-based interpretations.

Assay procedure of
6 hours
Assay procedure of
6 hours

Main characteristics

Test Principle

HYDRAGEL von WILLEBRAND MULTIMERS allows separating small, intermediate, and large molecular weight multimers.


The assay is carried out in two stages:

  • Sample pre-treatment followed by an electrophoresis done in SDS agarose gel to separate proteins according to their molecular weight,
  • direct immunofixation of multimers in the gel using anti-human vWF antibody followed by immunoenzyme staining to visualize the different multimers.


The semi-automated HYDRASYS 2 SCAN FOCUSING instrument performs all the steps needed to obtain gels ready for visual and densitometry-based interpretations.

Sample Type

Plasma samples

  • Excellent reproducibility
  • All multimer sizes
  • Densitometry helps for results interpretation
  • Patient’s profile overlayed with a normal control
Product details

Ready-to-use gels and reagents: 10 gels of 5 or 11 tests.


  • HYDRAGEL 5 von WILLEBRAND MULTIMERS kit – P/N: 4359 to be associated to Revelation kit – P/N: 4747
  • HYDRAGEL 11 von WILLEBRAND MULTIMERS kit – P/N: 4360 to be associated to Revelation kit – P/N: 4747 x2