HYDRAGEL 5 VON WILLEBRAND MULTIMERS

Simplified, same-day results, von Willebrand multimers gel assay

Test presentation

HYDRAGEL von WILLEBRAND MULTIMERS kit is designed for the detection of abnormalities in the VWF multimers distribution related to a hemorrhagic diathesis.
The test allows to assess the overall size distribution of von Willebrand factor multimers in human plasma using agarose gel electrophoresis and immunofixation, with the semi-automated the HYDRASYS 2 instrument.
HYDRAGEL von WILLEBRAND MULTIMERS assay provides within-day results, thanks to its simplified procedure, reduced hands-on time, ready-to-use reagents, and clear and reproducible profiles.

Test principle

HYDRAGEL von WILLEBRAND MULTIMERS allows separating small, intermediate and large multimers. The assay is carried out in two stages:

  • Sample pre-treatment followed by an electrophoresis done in SDS agarose gel to separate proteins according to their molecular weight,
  • direct immunofixation of multimers in the gel using anti-human vWF antibody followed by immunoenzyme staining to visualize the different Multimers.
    The semi-automated HYDRASYS 2 instrument performs all the steps needed to obtain gels ready for visual and densitometry-based interpretations.

Performances

  • Same-day results
  • Ready-to-use gels and reagents
  • Excellent reproducibility
  • Reduced hands-on time
  • All multimer sizes
  • Densitometry helps for results interpretation
  • Patient's profile overlayed with a normal control

Kit description

HYDRAGEL 5 von WILLEBRAND MULTIMERS kit (PN 4359): 10 gels of 5 tests to be associated to revelation kit (PN 4747)

Kit content:

  • Agarose gels
  • Buffered strips
  • Sample diluent
  • Wash solution
  • Intermediate wash solution
  • Rehydrating solution
  • Plastic masks
  • Thin filter Papers
  • Thick filter Papers

5 von WILLEBRAND MULTIMERS VISUALIZATION kit (PN 4747): 1 kit is necessary for 10 gels of 5 tests associated to electrophoresis kit (PN 4359)

Kit content:

  • Antiserum diluent
  • Anti-von Willebrand factor antiserum
  • Anti-Ig G - PER antibody
  • TTF1 / TTF2 solvent
  • TTF1 (stock solution)
  • TTF2 (stock solution)

Related products and accessories not provided in the kit:

  • HYDRASYS 5 VON WILLEBRAND MULTIMERS ACCESSORIES SEBIA (PN 1651)
  • METAL WEIGHT SEBIA (PN 1652) (1.8 KG)

Compatible Instruments

HYDRASYS 2

HYDRASYS 2 SCAN

HYDRASYS 2 SCAN FOCUSING

See all the posters and publications related to VWF in the SCIENTIFIC PUBLICATIONS section.

For Research Use Only.

Contact your Sebia representative for more details.