HYDRAGEL 18 A1AT ISOFOCUSING

The gold standard assay for the Alpha 1-antitrypsin (A1AT) phenotype testing

Test presentation

The HYDRAGEL 18 A1AT ISOFOCUSING kit used on the HYDRASYS FOCUSING instrument provides qualitative detection of the different phenotypes of the A1AT in human serum.

The semi-automated HYDRASYS instrument enables migration, immunofixation and direct detection on agarose gels, in order to obtain A1AT profiles for qualitative analysis.

Phenotyping results in conjunction with clinical findings and other laboratory assays aid in the diagnosis of Alpha 1-antitrypsin deficiency.

TEST PRINCIPLE

The HYDRAGEL 18 A1AT ISOFOCUSING assay is based on the principle of agarose gel isoelectrofocusing followed by immunofixation.

After the proteins separation according to their isoelectric point, the gel is directly incubated with a peroxydase labeled anti-A1AT with no need for a membrane blotting step.
Then, the gel is processed in order to remove unbound proteins before the enzymatic reaction.
Finally, the gel is visually evaluated.

Performances

Sensitivity
- Detection limit of an Alpha 1-antitrypsin’s isoform: 5 mg/dL.

Kit description

HYDRAGEL 18 A1AT ISOFOCUSING kit: 10 gels of 18 tests

HYDRAGEL 18 A1AT ISOFOCUSING (PN 4357)

Kit content:

Agarose gels
Ethylene glycol solution
Anodic and cathodic solutions
Strips
Sample diluent A1AT
Antiserum diluent A1AT
Wash solution A1AT
Rehydrating solution
TTF1 / TTF2 solvent
TTF1 and TTF2 reagents
Applicators
Buffer containers
Antiserum segments
Filter papers (thin and thick)

Related product and accessories not included in the kit:

Quality control: A1AT controls (MM, MZ and MS) (PN 4771)
Accessory kit for HYDRASYS A1AT ISOFOCUSING (PN 1253)
Anti-A1AT PER antiserum (PN 4746)
Destaining solution (PN 4540)
HYDRASYS wash solution (PN 4541)
Dynamic mask (PN 1255)

Compatible Instruments

HYDRASYS FOCUSING

HYDRASYS 2 FOCUSING

HYDRASYS 2 SCAN FOCUSING

NOTE: Contact your Sebia representative to verify the product availability in your country.