Sensitive immunological characterization of monoclonal free light chains

Test presentation

Protein immunofixation is a well-established technique for the characterization of any suspected monoclonal component on serum or urine electrophoresis.

The HYDRAGEL BENCE JONES, when used on the HYDRASYS instrument, separates and characterizes Bence Jones proteins (Monoclonal Free Light Chains) or any other full monoclonal immunoglobulin, in human urine or serum.

The semi-automated HYDRASYS instrument offers sample application, electrophoretic migration, immunofixation and staining.

The sensitivity of the HYDRAGEL BENCE JONES kit allows the detection of very small monoclonal components.

A recent development on GELSCAN provides the ability to quantify the monoclonal protein from the ELP track.


Test principle

The HYDRAGEL BENCE JONES assay is based on the principle of agarose gel electrophoresis immunofixation.

The HYDRASYS system is a semi-automated multi-parameter instrument.

The analytical steps include processing of the agarose gel in the following sequence:

  • Sample application
  • Electrophoretic migration
  • Immunofixation
  • Staining with acid violet dye
  • Destaining
  • Final drying

After separating serum or urine proteins according to their charge, the gel is incubated with different specific antisera targeted against:

  • Heavy chains Gamma, Alpha, Mu (using the trivalent antiserum)
  • Kappa (κ) and Lambda (λ) free and bound light chains
  • Kappa (κ) and Lambda (λ) free light chains

The gel is subsequently washed in order to remove unbound antisera excess prior to the final staining step. The gels are then interpreted visually. Bands present on the immunological tracks indicate the presence of monoclonal immunoglobulin and/or free light chains.


  • Sensitivity:
    • Internal sensitivity study demonstrated that a Bence Jones protein can be detected in a serum at a concentration:
      • as low as 20 mg/L with anti-free & bound light chains antisera
      • as low as 50 mg/L with anti-free light chains antisera.
  • Full traceability and positive ID is obtained by connecting the HYDRASYS to the ASSIST pipetting station

Kit description

HYDRAGEL BENCE JONES kit: 10 gels of 1, 2 or 4 tests

  • HYDRAGEL 1, 2, 4 BENCE JONES (SM*) (PN 4821, 4822, 4824)
  • HYDRAGEL 1, 2, 4 BENCE JONES (DM**) (PN 4321, 4322, 4324)

Kit content:

  • Agarose gels  
  • Buffered strips
  • Acid violet stain
  • Applicators
  • Filter papers (thick and thin)
  • Filter paper combs*
  • Antisera segments**

Related products and accessories not provided in the kit:

  • Quality controls:
    • IT/IF control (PN 4788)
    • Normal control serum (PN 4785)
  • Dynamic mask (PN 1255)
  • Accessory kit for HYDRASYS IF (PN 1260)
  • Accessory kit for HYDRASYS 1 IF / 1 BENCE JONES (PN 1267)
  • HYDRAGEL IF sample diluent (PN 4588)
  • (GAM, K, L) antisera and fixative solution kit (SM*) (PN 4835)
  • (GAM, K, L) antisera and fixative solution kit (DM**) (PN 4335)
  • Antisera K & L free light chains (SM*) (PN 4836)
  • Antisera K & L free light chains (DM**) (PN 4336)
  • Destaining solution (PN 4540)
  • HYDRASYS wash solution (PN 4541)
  • FLUIDIL (PN 4587)

Compatible Instruments









* Only with Sebia’s standard mask (SM)

** Only with Sebia’s dynamic mask (DM)

NOTE: Contact your Sebia representative to verify the product availability in your country.