MINICAP HEMOGLOBIN(E)

HEMOGLOBIN ANALYSIS BY CAPILLARY ELECTROPHORESIS: A CLEAR PATTERN FOR EASY INTERPRETATION

TEST PRESENTATION

Hemoglobin electrophoresis is a well-established technique routinely used in clinical laboratories to screen samples for hemoglobinopathies (hemoglobin variants and thalassemias).

The MINICAP HEMOGLOBIN(E) kit enables, on MINICAP instruments, the efficient separation of hemoglobin fractions and detection of a large number of hemoglobin variants and thalassemias patterns.

MINICAP instruments perform all sequences automatically, from sampling to the final clear-cut profile, with precise quantification and presumptive identification of the most common hemoglobins.

MINICAP instruments allow high resolution separation of the major hemoglobin variants (Hb S, Hb C, Hb D and Hb E) and accurate quantification of the Hb A2 and Hb F.

Hb H and Hb Bart’s, as seen in alpha-thalassemia are clearly separated and easily quantified.

The MINICAP HEMOGLOBIN(E) kit allows hemoglobin analysis on adult samples.

 

TEST PRINCIPLE

The MINICAP HEMOGLOBIN(E) assay is based on the principle of capillary electrophoresis in free solution. Hemoglobin fractions are separated in silica capillaries, by their electrophoretic mobility and electroosmotic flow at a high voltage in an alkaline buffer. Hemoglobin fractions are directly detected at the specific absorbance of 415 nm. The pattern is divided into 15 zones. Each zone displays a drop down library of possible variants migrating within this zone. More than 300 variants are listed.

MINICAP HEMOGLOBIN(E) users can request from their Sebia representative the HEMOGLOBINS ATLAS CD for more information.

 

PERFORMANCES

  • Throughput: 8 tests/h
  • Excellent precision and linearity on Hb A, Hb F, Hb A2 and common variants measurements
  • Full traceability and positive ID for samples and reagents
  • MINICAP FLEX PIERCING: cap piercing capacity and whole blood primary tube mixing

KIT DESCRIPTION

MINICAP HEMOGLOBIN(E) (PN 2207) kit content:

  • Buffer
  • Hemolyzing solution
  • Wash solution
  • Reagent cups
  • Filters
  • Bins for used cups​

Related products and accessories not provided in the kit:

  • Quality controls:
    • Normal Hb A2 control (PN 4778)
    • Pathological Hb A2 control (PN 4779)
    • Hb AFSC control (PN 4792)
  • Tubes and caps for control (PN 9202, 9205)
  • Automatic low volume bar code labels (PN 9208)
  • Manual low volume bar code labels (PN 9209)
  • CAPICLEAN (PN 2058) - only for MINICAP instrument
  • CAPICLEAN MINICAP (PN 2251) - only for MINICAP FLEX-PIERCING instrument
  • HEMOGLOBINS ATLAS
  • CAPIprotect (2 x 5L) (PN 2061)

Compatible Instruments

NOTE: Contact your Sebia representative to verify the product availability in your country.