Presence of intrathecal IgG suggests inflammatory disease of the Central Nervous System, such as caused by multiple sclerosis (MS).
The HYDRAGEL CSF ISOFOCUSING kit used on the HYDRASYS 2 SCAN FOCUSING instrument provides qualitative detection and immunological characterization of IgG oligoclonal banding in human CSF (Cerebro Spinal Fluid) and serum. The semi-automated HYDRASYS instrument enables migration, immunofixation and direct detection on agarose gels, in order to obtain IgG profiles for qualitative analysis.
The main recommendations of European Consensus Report for Multiple Sclerosis diagnosis are:
The presence of CSF oligoclonal bands only, characterizing local IgG synthesis, tightly correlates with the diagnosis of Multiple Sclerosis. Their presence provides useful information when in agreement with the other clinical investigations.
Sensitivity increased about a 100-fold compared to standard immunofixation thanks to the detection with enzyme labelled antibodies.
Clear oligoclonal band visualization for a reliable interpretation.
Simple step-by-step procedure without membrane transfer for 3 hours of turnaround time.
HYDRAGEL CSF ISOFOCUSING meets all requirements needed to objectively detect CNS local IgG synthesis in multiple sclerosis.
The HYDRAGEL CSF ISOFOCUSING assay is based on the principle of agarose gel isoelectrofocusing followed by immunofixation. The patient’s serum and CSF samples are analysed in parallel to compare the IgG distribution.
Serum and CSF samples are performed in parallel for each patient to compare IgG distribution as recommended by the European consensus.
Flexible Format of HYDRAGEL CSF ISOFOCUSING kit: 10 gels of 3 or 9 tests.
Large menu with high quality results on gel electrophoresis.
IFCC Euromedlab 2021
Sebia booth: #121
Sebia Workshop: EduW22
IFCC Worldlab 2022
Sebia booth: #72
Sebia Workshop: EduW25
2017 Mc Donald Criteria for multiple sclerosis diagnosis includes CSF IgG oligoclonal bands.
2005 Multiple sclerosis consensus criteria includes CSF IgG oligoclonal bands.
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